Abstract: Objective　To study the correlation with Genes Coding for ESBLs and Class Ⅰ Integron in ESBLs-producing Escherichia coli from children. Methods　PCR was used for gene typing detection of 100 strains of ESBLs-producing Escherichia coli strains. While detection of class I integrase gene in the 100 strains ESBLs-producing Escherichia coli and 100 strains of non-ESBLs producing Escherichia coli were separately performed by PCR. It provides the solid base not only to reveal the relationship between class I integron and drug resistance, but also the relationship between class I integron and ESBLs-producing. Results　The most frequently genotyping from ESBLs-producing Escherichia coli in children is CTX-M (84%), followed by TEM-1 (63%). The predominant distribution of genotype in ESBL- producing strains is TEM-1 + CTX-M (45%), followed by CTX-M (34%). Class I integrase gene detected in ESBLs- producing and non- ESBLs producing strain were 100 cases (100%) and 25 cases (25%) separately, the difference was statistically significant (P<0.05); drug resistance in class I integron positive strains were significantly higher than in class I integron negative strains, especially in Ciprofloxacin, Levofloxacin, and Aminoglycoside (86.4%, 88%, and 80%). Conclusions　The distribution of Class I integron in ESBLs-producing Escherichia coli is significantly higher than that in non-ESBLs-producing strains, It is rational that Class I integron highly correlate with strong drug resistance in ESBLs-producing strains.